Adventure Island is a water park located northeast of Tampa, Florida, across the street from Busch Gardens Tampa Bay. The park features 30 acres (12 ha) of water rides, dining, and other attractions. The park opened on June 7, 1980, and is part of SeaWorld Entertainment. Fun in the sun featuring the ultimate combination of high-speed thrills and tropical, tranquil surroundings for guests of all ages. Race down rapids, dig your toes into the sand or relax all day in a private cabana. New at Adventure Island, hurtle high and experience a feeling of weightlessness on Colossal Curl™, Adventure Island’s newest family thrill slide.

Busch Gardens Tampa Bay is a 335-acre African-themed animal theme park located in the city of Tampa, Florida. Busch Gardens Tampa is owned and operated by SeaWorld Parks & Entertainment and is the 2nd largest park in terms of attendance behind SeaWorld Orlando. The park officially features nine roller coasters and two water rides amongst other rides and animal attractions. Alongside sister waterpark Adventure Island, Busch Gardens is the anchor of Tampa's 365-acre amusement multiplex. Currently Busch Gardens competes with other such parks in Florida and charges comparable fees. Busch Gardens is currently accredited by the Association of Zoos and Aquariums (AZA). In 2015, the park hosted 4.2 million people, placing 11th as the most-visited theme parks in the United States.

The Museum of Science & Industry (MOSI) is a not-for-profit science museum located in Tampa, Florida. It is a community-based institution and educational resource that is dedicated to advancing public interest, knowledge, and understanding of science, industry, and technology. MOSI's core ideology is to make a difference in people's lives by making science real for people of all ages and background. MOSI's funding comes from private donations, corporate sponsors, and support from Hillsborough County and the City of Tampa. MOSI began in 1962 when Hillsborough County first approved funding for a youth museum in Sulphur Springs. Named the Museum of Science and Natural History, it provided natural science exhibits and education programs to children and adults. The name of the museum was changed to the Hillsborough County Museum in 1967. In 1976, the Hillsborough County Museum's advisory committee and staff started construction on a new museum in North Tampa that was to become the Museum of Science & Industry. The museum was completed in 1980 and permanently opened to the public on January 23, 1982.

The Tampa Museum of Art is located in downtown Tampa, Florida. It exhibits modern and contemporary art, as well as Greek, Roman, and Etruscan antiquities. The museum was founded in 1979 and debuted an award-winning new building in 2010 just north of its original site along Tampa's Riverwalk on the banks of Hillsborough River. Since its inception, museum planners knew that the Tampa Museum of Art's original building was too small for its collection. Proposals for expansion or relocation were the subject of discussion and controversy for years. Several different plans were proposed either by the city of Tampa or the museum board.

The Florida Aquarium is a 501(c)(3) not-for-profit organization, publicly operated institution located in Tampa, Florida, United States. It is a large scale, 250,000-square-foot aquarium and is accredited by the Association of Zoos and Aquariums. This means they are a leader in conservation and education, supporting programs for wildlife and having a strong educational component in the forms of summer camps, school trips, etc. The facility is home to more than 7,000 aquatic plants and animals from Florida and all over the world. The facility is located in the Channel District of Downtown Tampa. The Florida Aquarium opened in March 1995 as a privately funded entity and became a public-private partnership when the city of Tampa assumed responsibility for its debt in 1999. On April 18, 2012, the AIA's Florida Chapter placed the Florida Aquarium on its list of Florida Architecture: 100 Years. 100 Places.

ZooTampa at Lowry Park (formerly known as Lowry Park Zoo) is a 63-acre nonprofit zoo located in Tampa, Florida. In 2009, Lowry Park Zoo was voted the #1 Family Friendly Zoo in the US by Parents Magazine, and is recognized by the State of Florida as the center for Florida wildlife conservation and biodiversity. The zoo is operated by the Lowry Park Zoological Society, an independent 501(c)(3) charitable organization. The zoo also exists as a center for conservation of endangered wildlife both locally and around the globe. Tampa’s Lowry Park Zoo is accredited by the Association of Zoos and Aquariums (AZA) as well as a member of the World Association of Zoos and Aquariums (WAZA), Conservation Breeding Specialist Group (CBSG), the Florida Association of Zoos and Aquariums (FAZA) and the Florida Attractions Association (FAA). ZooTampa traces its origins to a small animal exhibit that was located on the grounds of the University of Tampa in downtown. It moved to its current location as the Lowry Park Zoo in 1957, and was completely rebuilt and reconfigured in 1989. The zoo rebranded itself as ZooTampa at Lowry Park in 2018 as part of another renovation and expansion project.